High Purity L-Isoleucine Powder | ≥98.5% Feed, Food & Supplement Grade | CAS 73-32-5
Description:
Product Overview

L-Isoleucine is an essential branched-chain aliphatic amino acid that cannot be synthesized by the body and must be obtained through diet or supplementation. It is one of the three branched-chain amino acids (BCAAs), alongside L-Leucine and L-Valine, which are unique in that their catabolism initiates in muscle tissue and yields NADH and FADH₂ that can be utilized for ATP generation.
As a hydrophobic amino acid with a branched side chain, L-isoleucine plays a fundamental role in protein biosynthesis, hemoglobin formation, and the regulation of blood sugar and energy levels. It is particularly important for muscle metabolism, endurance, and recovery after exercise, as it is actually broken down for energy within muscle tissue. In addition to its role in protein synthesis, L-isoleucine stimulates immune function, promotes the secretion of several hormones, assists wound healing, and participates in detoxifying nitrogenous wastes.
The addition of L-isoleucine in animal feed has been shown to improve feed conversion rates, allowing for the replacement of soybean meal and reduction of dietary crude protein levels. This makes it a valuable feed additive in poultry, swine, and aquaculture nutrition.
At Henan Liyin Biotech, we supply high-purity L-Isoleucine in the form of a white to yellowish crystalline powder, with a minimum purity of ≥98.5%, compliant with USP, EP, and FCC standards. Our product is available in Feed Grade, Food Grade, and Supplement Grade to serve applications across animal nutrition, human food, dietary supplements, and personal care.
Why Choose Our L-Isoleucine?
Verified Purity: Assay ≥98.5% (typically 98.5–101.0%), ensuring consistent potency for all applications
Multi-Standard Compliance: Meets USP, EP, and FCC specifications, providing global regulatory acceptance
Proven Safety Profile: Essential amino acid with well-established safety for human and animal nutrition
Complete Traceability: Each batch is accompanied by a comprehensive Certificate of Analysis (COA)
Flexible Packaging: Available in packaging options from 1kg to bulk quantities
Key Functional Properties

BCAA & Muscle Metabolism
L-Isoleucine is one of the three branched-chain amino acids, which are unique because their initial catabolism takes place in muscle rather than the liver. This makes BCAAs particularly important for muscle energy production, with isoleucine being broken down for energy within muscle tissue. It supports endurance, helps repair and rebuild muscle, and promotes recovery after exercise.
Hemoglobin Formation & Blood Sugar Regulation
L-Isoleucine is important for hemoglobin synthesis and plays a role in regulating blood sugar and energy levels. It is involved in the formation of hemoglobin, the molecule responsible for transporting oxygen in the blood.
Immune Support & Hormone Secretion
This essential amino acid stimulates immune function and promotes the secretion of several hormones. It is also used in the body for detoxifying nitrogenous wastes and assisting wound healing.

Metabolic Role
L-Isoleucine participates in protein synthesis by binding with transfer RNA (tRNA) in the cell cytoplasm. It serves as a metabolite for several species, including Saccharomyces cerevisiae, Escherichia coli, plants, algae, and mice. The catabolism of isoleucine terminates with production of acetylCoA and propionylCoA, making it both glucogenic and ketogenic.
Quality Assurance & Documentation

All batches undergo rigorous quality control, including:
Assay by HPLC: ≥98.5% (typically 98.5–101.0%)
Heavy Metal Screening: Lead ≤ 5.0 mg/kg, Arsenic ≤ 2.0 mg/kg
Microbiological Testing: For safety and purity
Specific Rotation and pH: Verified against USP/EP/FCC specifications
Loss on Drying and Residue on Ignition: To ensure stability and purity
We provide complete documentation with every shipment, including Certificate of Analysis (COA), Material Safety Data Sheet (MSDS), and Allergen Statement. Our GMP-certified production ensures batch-to-batch consistency and traceability.
Sourcing Support
We offer flexible packaging and can provide samples for pre-shipment testing. Our technical team is available to assist with grade selection (Feed, Food, or Supplement Grade), application guidance, and custom formulation support — from R&D trials to full-scale commercial production.
Contact us to discuss your specific L-Isoleucine requirements or to request samples for your formulations.
Parameters:
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Parameter
Specification
Product Name
L-Isoleucine
CAS Number
73-32-5
Molecular Formula
C₆H₁₃NO₂
Molecular Weight
131.17 g/mol
Assay (Purity)
≥ 98.5% (typically 98.5–101.0%)
Appearance
White crystals or crystalline powder
Available Grades
Feed Grade, Food Grade, Supplement Grade
Standards
USP, EP, FCC
Specific Rotation
[α]²⁰/D +38.9° to +41.8°
pH (1g/100mL)
5.5 – 7.0
Loss on Drying
≤ 0.3%
Residue on Ignition
≤ 0.3%
Heavy Metals
≤5.0 mg/kg
Arsenic (As)
≤ 2.0 mg/kg
Melting Point
288 °C (dec.) (lit.)
Solubility
1 M HCl: 50 mg/mL
Packaging Options
1kg, 5kg, 25kg (or as per request)
Documentation
Full COA, MSDS, Allergen Statement
Storage Conditions
Store in a cool, dry place, protected from light and moisture
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L-Isoleucine is an essential branched-chain amino acid (BCAA) that plays a critical role in muscle metabolism, endurance, and recovery. As one of the three BCAAs, it is broken down for energy within muscle tissue and supports hemoglobin formation, blood sugar regulation, and immune function. Available in Feed, Food, and Supplement Grades with ≥98.5% purity, backed by full documentation and batch-to-batch consistency.
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Dietary Supplements

L-Isoleucine is a popular ingredient in supplements targeting sports nutrition, muscle recovery, and endurance. As one of the three BCAAs, it is particularly valuable for athletes and active individuals.
Key Applications:
- Sports Nutrition: Supports endurance, muscle repair, and recovery after exercise
- BCAA Formulations: Often combined with L-Leucine and L-Valine for comprehensive branched-chain amino acid support
- Energy Production: Broken down for energy within muscle tissue during exercise
- Hemoglobin Support: Important for the formation of hemoglobin, which transports oxygen in the blood
- Typical Dosage: Supplementation levels vary by intended use. L-Isoleucine is generally recognized as safe when taken as directed.
Food & Beverage

L-Isoleucine is used in the food industry as a nutritional supplement and flavoring agent.
Key Applications:
- Food Fortification: Used to enhance the amino acid profile of processed foods and protein-based products
- Sports Nutrition Products: Used in amino acid nutraceuticals, sports and fitness nourishment, and amino acid functional beverages
- Flavoring Agent: Approved for use as a flavoring agent in foods
- Regulatory Status: FCC grade is suitable for food, beverage, and nutritional supplement applications. L-Isoleucine is recognized as GRAS when properly used. FDA regulations include 21 CFR 172.320 for use as a nutrient supplement.
Animal Feed

L-Isoleucine is an essential feed additive for poultry, swine, and aquaculture. The addition of L-isoleucine to animal feed has been shown to improve feed conversion rates, allowing for the replacement of soybean meal and the reduction of dietary crude protein levels.
Key Applications:
- Poultry: Supports growth performance and feed efficiency
- Swine: Essential for growth and development in pig diets
- Aquaculture: Used in fish and shrimp feeds to support growth
- Improved Feed Conversion: Reduces dietary crude protein requirements while maintaining performance
Recommended Inclusion: Levels vary by species and production stage. Our Feed Grade L-Isoleucine is formulated for optimal dispersion in premixes and pelleted feeds.
Personal Care & Cosmetics
L-Isoleucine is used in personal care formulations for its skin-conditioning and hair-conditioning properties.
Key Applications:
- Skin Care: Supports skin health through its role in cellular metabolism
- Hair Care: Used in hair conditioning products
- Formula Compatibility: Stable in topical formulations and compatible with a wide range of cosmetic ingredients
- Sports Nutrition: Supports endurance, muscle repair, and recovery after exercise
Key words:
High Purity L-Isoleucine Powder | ≥98.5% Feed, Food & Supplement Grade | CAS 73-32-5
Function:
L-Isoleucine is an essential branched-chain amino acid (BCAA) that plays a critical role in muscle metabolism, endurance, and recovery. As one of the three BCAAs, it is broken down for energy within muscle tissue and supports hemoglobin formation, blood sugar regulation, and immune function. Available in Feed, Food, and Supplement Grades with ≥98.5% purity, backed by full documentation and batch-to-batch consistency.
Application:
Dietary Supplements

L-Isoleucine is a popular ingredient in supplements targeting sports nutrition, muscle recovery, and endurance. As one of the three BCAAs, it is particularly valuable for athletes and active individuals.
Key Applications:
- Sports Nutrition: Supports endurance, muscle repair, and recovery after exercise
- BCAA Formulations: Often combined with L-Leucine and L-Valine for comprehensive branched-chain amino acid support
- Energy Production: Broken down for energy within muscle tissue during exercise
- Hemoglobin Support: Important for the formation of hemoglobin, which transports oxygen in the blood
- Typical Dosage: Supplementation levels vary by intended use. L-Isoleucine is generally recognized as safe when taken as directed.
Food & Beverage

L-Isoleucine is used in the food industry as a nutritional supplement and flavoring agent.
Key Applications:
- Food Fortification: Used to enhance the amino acid profile of processed foods and protein-based products
- Sports Nutrition Products: Used in amino acid nutraceuticals, sports and fitness nourishment, and amino acid functional beverages
- Flavoring Agent: Approved for use as a flavoring agent in foods
- Regulatory Status: FCC grade is suitable for food, beverage, and nutritional supplement applications. L-Isoleucine is recognized as GRAS when properly used. FDA regulations include 21 CFR 172.320 for use as a nutrient supplement.
Animal Feed

L-Isoleucine is an essential feed additive for poultry, swine, and aquaculture. The addition of L-isoleucine to animal feed has been shown to improve feed conversion rates, allowing for the replacement of soybean meal and the reduction of dietary crude protein levels.
Key Applications:
- Poultry: Supports growth performance and feed efficiency
- Swine: Essential for growth and development in pig diets
- Aquaculture: Used in fish and shrimp feeds to support growth
- Improved Feed Conversion: Reduces dietary crude protein requirements while maintaining performance
Recommended Inclusion: Levels vary by species and production stage. Our Feed Grade L-Isoleucine is formulated for optimal dispersion in premixes and pelleted feeds.
Personal Care & Cosmetics
L-Isoleucine is used in personal care formulations for its skin-conditioning and hair-conditioning properties.
Key Applications:
- Skin Care: Supports skin health through its role in cellular metabolism
- Hair Care: Used in hair conditioning products
- Formula Compatibility: Stable in topical formulations and compatible with a wide range of cosmetic ingredients
About Us
Henan Liyin Biotech Co.,Ltd
We are is established in 2024,located in Zhengzhou,on the banks of the Yellow River-the revered “Mother River “of China .As a specialized supplier of human nutrition and animal health products ,the company focuses on a range of products including vitamins ,amino acids ,plant extracts ,and sweeteners,which are widely used in industries ,such as food ,health supplements,cosmetics ,feed and veterinary medicine .By integrating global product resources with the needs of international clients ,Liyin Biotech provides premium products and professional services tailored to each customer .
FAQ
How to contact ?
You can contact with us by Whatsapp:+86 15824830539, the Average Response Time is 0-4h on Work time, <24h on Non-worktime.
For every inquiry, our sales will provide the one-for-one service for you.
How about the payment terms ?
Normal orders: Company account ;Sample/small orders: Paypal(The 5-10% extrafee).About the payment term, we will negotiate alone.
How to get the samples ?
At the first time ,we can provide you the normal product <30g free sample
How to confirm the Product Quality before placing orders?
1st, you can get free samples for some products, just paythe basic cost for us.
2nd,Also you can send the specification or your quest tous, we will customize the products for you.3rd,We emphasis that our whole company make a livingwith organic chemical and customers, we absolutelyimpossible to sale any fake goods, unqualified goods, it isshame! And None False certificate/lie/illegal thing in oursales net!
Which kind of payment do you accept?
- As usually we use company account ,T/T in advance ,or at least 30% T/T in advance ,the balance can be paid by the copy of B/L
- For some countrys we can accept L/C at sight & DP at sight
- For smaples we can use Paypal (The 5-10% extra fees) or other ways
About the payment term, we will negotiate alone.
How about the shipment ?
1>For bulk goods ,we can use contaioner by sea ,1&20 and 1&40 contaioner
2>For urgently and expensive goods ,we can arrange the air lines for customers choose ,or can as customer required
3>For samples or small quantity goods ,we can use Fedex or other express ways
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